Fig. 9

Immunoblotting of fibrinogen in the ipsilateral hemisphere and quantification of intracerebral hemorrhage. \(\beta\)-actin was used for immunoblotting housekeeping genes (normal saline treatment for sham; Rose Bengal (RB) with thrombin treatment for T+RB; rtPA treatment for rtPA; rmDPPs with magneto-sonothrombolytic approach for +M+US group). (a-b) Typical immunoblotting and quantification of fibrinogen in the RB and T+RB groups, showing stable platelet deposits were observed in the ipsilateral hemisphere compared with RB alone (p = 0.047). (c-d) Typical immunoblotting and quantification of fibrinogen expression in the sham, T+RB, rtPA, and +M+US groups, showing that rtPA treatment showed not only an unstable fibrinolytic potential (standard deviation: 0.507, \(n=5\)), but also no significant difference was detected when compared to the T+RB (T+RB for \(n=12\); rtPA for \(n=3\); \(p=0.16\)). There was no significant difference between the rtPA and +M+US groups (\(p=0.80\)). (e) Typical image of intracerebral HT after delayed treatment with either drug or particles with a magneto-sonothrombolytic approach. (f-g) Regressed cyanmethemoglobin standard curve and quantification of intracerebral HT, showing delayed treatment of rtPA induced the HT (\(n=4\)), whereas the +M+US group did not present any HT (\(n=3\))