Fig. 6

Cytotoxic T cell responses of murine splenocytes stimulated with npGG aAPCs A T cell activation profile of freshly isolated splenocytes stimulated with activator-npGG over 24 h, 3 d and 7 d. Activated cells were gated as CD45⁺CD4⁺CD69⁺ and CD45⁺CD8⁺CD69⁺ T cells on a BD FACSAria™ III. Summarized data is shown as bar graphs. B Flow cytometric plots of intracellular staining and frequencies of expression of Perforin (i) and Granzyme B (ii) in CD45⁺CD8⁺CD69⁺ T cells are shown. Quantitative results are expressed as the mean ± standard deviation where n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, two-way ANOVA with Tukey multiple comparison post-test