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Fig. 3 | Biomaterials Research

Fig. 3

From: Oxygen tank for synergistic hypoxia relief to enhance mitochondria-targeted photodynamic therapy

Fig. 3

Prolonged circulation and mitochondria-targeted ability of Oxygen Tank. a Schematic illustration of the prolonged circulation by RBCm-coating NPs. b The clearance rate of the Oxygen Tank is determined by flow cytometry. RAW264.7 cells were treated with the Oxygen Tank or AIP for different lengths of time (0 h, 1 h, 2 h, and 4 h). c Subcellular localization of Oxygen Tank compared to lysosome and mitochondria trackers determined by CLSM. The scale bar is 10 μm. PC means the Pearson Correlation coefficient. d. Colocalization analysis of Oxygen Tank in AGS cells with lysosome tracker. e Colocalization analysis of Oxygen Tank in AGS cells with mitochondria tracker. f Mitochondria potential evaluation by JC-1 after AGS cells treated with PBS, ATO, IP NPs, and Oxygen Tank NPs. Red signals (JC-1 aggregates) suggested a normal polarized mitochondrial membrane. Green signals (JC-1 monomers) suggested an abnormal depolarized mitochondrial membrane. The scale bar is 50 μm

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