Fig. 1

Schematic protocol indicates decellularization process using SDS, TX, and CHAPS detergents. Perfusion process was performed at room temperature and lasts 16 hours, while shaking process was performed at 37 °C (A) Gross view of NRA and DRA tissue decellularized using 0.25% SDS + 0.5% TX is shown in Fig. 1 indicating tissue transparency following the decellularization process (B). NRA: Native Rat Aorta, DRA: Decellularized Rat Aorta, SDS: Sodium Dodecyl Sulphate, TX: Triton X-100, CHAPS: 3-[(3-cholamidopropyl)