Fig. 5
MgH2 regulated inflammation, apoptosis, pyroptosis and cell cycle progression by suppressing oxidative stress. ESR assays were used to measure the ability of different concentrations of MgH2 to scavenge the [·OH] generated by the Fenton reaction (A), and the peak value of the ESR spectrum was recorded (B). The hydrogen microelectrode was performed to monitor the hydrogen distribution in the testes of mice(C). ROS generation in GC2 cells irradiated with 10 Gy was detected by flow cytometry(D), and the positive rates were recorded (E). Subsequently, the levels MDA (F) in the testes 12 hours after 5 Gy irradiation were detected, and the effect of MgH2 on the level of apoptosis at 12 h after IR was evaluated. The expression of apoptosis-related proteins in testes (G) and GC2 cells (H) was detected. The testis tissue was stained for Tunel assay (I), and the number of Tunel-positive cells in each seminiferous tubule was counted (J). The effect of MgH2 on cell cycle-related protein expression in GC2 cells was also detected after MgH2 administration alone (K) and 48 h (L) after irradiation. The data are expressed as the mean ± SEM, * p < 0.05, ** p < 0.01