Fig. 3
MgH2 improved the decrease in sperm motility and quality induced by ionizing radiation. Changes in sperm count and motility were evaluated on the 29th day after 5 Gy whole body irradiation. CASA was used to detect the motility of the sperm from murine epididymis tissues (A). The counts of sperm (B) and morphological characteristics (D) of mouse sperm were observed under a microscope, and the deformity rate (C) was assessed (n = 200). Then, the genetic effect of MgH2 was evaluated in IR-treated mice. After 5 Gy whole body irradiation for 21 days, the number of pups (E) and the number of deaths (F) per litter were recorded. In addition, changes in the chromosomes of testes in F0 generation mice (H) and bone marrow in F1 generation mice (I) were also observed under as use Chromosome image analysis system observed under Metafer Automated Slide Scanning Platform. Moreover, the human sperm movement was captured in the diagram (G). The data are expressed as the mean ± SEM, *p < 0.05, **p < 0.01