Fig. 12

Histological evaluation of regenerated nerves at 4 weeks. A H&E staining and immunofluorescence staining were performed at middle and distal segment separately. Anti-Neurofilament 200 was used to stain regenerative axons (red) and anti-S100 antibody was used to stain regenerative myelin sheath (green). Nuclei were stained with DAPI (blue). Statistical analyses were performed by calculating B-C the mean density of regenerative axons between groups. ^*P < 0.05, ^**P < 0.01, and n.s. not significant. Micro-T microtissues, PRP platelet-rich plasma