Fig. 1

Production of M2-like macrophages by M-CSF + IL-4 treatment. A The cell morphology was shown under the light microscopy. M0 macrophages (without M-CSF plus IL-4 treatment) were used as controls. Scale bar 100 μm. B The surface expression of CCR2 and CD206 was measured using flow cytometry. Below was the fluorescent intensity of CCR2 and CD206, and the quantification results. C The gene expression of M1- and M2-related markers, including IL-1β, TNF-α, iNOS and IL-10, TGF-β, Arg-1, were measured by qPCR. (Data were presented as mean ± SD. ***p < 0.001. N = 3 per group)