Fig. 3

Uptake and distribution of SCNTs/FAM-si-circPRMT5 by bladder cancer cells. (A) Images of SCNTs/FAM-si-circPRMT5 fluorescence at 6 h post-transfection acquired using fluorescence and bright field microscopy. (B) Cell uptake of SCNTs/FAM-si-circPRMT5 with different siRNA contents by flow cytometry. Representative histograms are shown in the upper panel, the lower panel shows the mean ± SD (n = three independent experiments). (C) Confocal laser scanning microscopy (CLSM) images of the uptake of SCNTs/FAM-si-circPRMT5 by T24 cells at 1, 2, 4, and 8 h. FAM, green signal; DAPI (4′,6-diamidino-2-phenylindole), blue signal; Scale bar = 25 μm. (D) Representative confocal microscopy image showing the co-localization of SCNTs/FAM-si-circPRMT5 (green) with endocytic markers (dextran, cholera toxin, and transferrin) labeled with Alexa Fluor 555 (red). Hoechst 33342 (blue) was used to counterstain the nuclei (upper panel). Pearson’s correlation coefficient analysis of The co-localization between endocytosis markers and SCNTs/FAM-si-circPRMT5 in T24 cells using Pearson’s correlation coefficient analysis in Image J Coloc2 (lower panel). **P < 0.01