Fig. 1
Characterization of PEGylated prodrug-loaded nanoparticles. (A) The nanococktail from coassembling platinum (IV) and SN38 derivatives was prepared in an aqueous solution for intravenous injection. (B) TEM images of Pt(IV)-NP, SN38-NP, and the nanoparticle cocktail (the molar ratio of Pt-LA2 to SN38-LA prodrugs was fixed at 5:1). Scale bars, 100 nm. (C-E) Distribution of nanoparticle sizes (C), average hydrodynamic diameters and PDI (D), and zeta potentials (E), determined by DLS measurement. (F) Characterization of prodrug-formulated nanoparticles. (G-I) Assessment of nanoparticle stability in PBS (pH = 7.4) (G), in PBS supplemented with 20% fetal bovine serum (FBS) (H), or in PBS supplemented with 20% rat serum (I) over one week