Fig. 6

Human platelet-rich plasma (PRP) infusion repairs the damaged endometrium and improves implantation outcomes in Asherman’s syndrome (AS) mice [58]. (A) PRP was injected only into the right horn at 7 days after inducing injury to the bilateral uterine horns. At 14 days, some mice were sacrificed, and the uterine horns were prepared for analysis. The other mice were mated with fertile, healthy males. (B) HE staining of the endometrial tissues to evaluate morphologic structures. (C) Masson’s trichrome staining to evaluate collagen deposition (blue). (D) Immunofluorescence staining of collagen type 1A (Col1a1). (E) PCR results and (F) analyses of the expression of fibrosis-related factors (Col1a1, Tgfβ1, and Timp1). (G) Gross morphology of the implantation sites (IS) in AS mice with or without PRP. (H) The number of ISs in the PRP-treated horns of AS mice. (I) Photographs of embryos isolated from the IS on Day 12 of pregnancy. (J) A graph of the weights of IS between the AS group and the PRP-treated AS group on Day 12 of pregnancy. (B-D, up and down bar: 100 and 200 μm. Reprinted with permission from Ref. [58]