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Fig. 13 | Biomaterials Research

Fig. 13

From: Recent trends in therapeutic strategies for repairing endometrial tissue in intrauterine adhesion

Fig. 13

Enhanced differentiation potential of primary human endometrial cells cultured on three-dimensional (3D) scaffolds [118]. (A) The schematic diagram of the 3-D printer. (B) (a) Photographs of polyHIPE monoliths, (b) discs of 400 μm thickness, and (c) inserted in the cell culture well plate. (d) Presentation of a fully submerged polyHIPE scaffold in culture media using a Transwell insert. (C) Laser scanning confocal micrographs of cultured HEECs (a) in 2D; (b) in polyHIPE scaffold and HESCs (c) in 2D; and (d) in polyHIPE scaffold. Immunofluorescence staining of HEECs by anti-CK18 antibody/DAPI and HESCs by antivimentin antibody/DAPI. Bars: 5 μm. HE staining of HEECs (D) and HESCs (E) growth in polyHIPE scaffolds using 24-well plate format: (a, d) Day 2; (b, e) Day 7; (c, f) Day 15. Scaffolds were either left uncoated (a − c) or coated with fibronectin 5 (d − f). Fibronectin coating modestly promotes cell adhesion and migration into the material. Bars: 50 μm. Reprinted with permission from Ref. [118]

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