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Fig. 5 | Biomaterials Research

Fig. 5

From: Polyphenols-loaded electrospun nanofibers in bone tissue engineering and regeneration

Fig. 5

Analyses of in vitro cell viability and calcium deposition of catecholamine containing collagen nanofiber. A Electrospun nanofibrous mat was prepared from the composite of collagen (8% w/v), dopamine (10% w/w of collagen), and 20 mM CaCl2 in 90% HFIP. The brown coloration in the mats is due to the formation of polydopamine by electrochemical oxidation. Intensified brown color in mat and precipitation of CaCO3 occurred by the subsequent exposure of the mat to (NH4)2CO3 vapors. The nanofibrous mat exhibited excellent mechanical properties, surface wettability, fluorescence, and osteoblast cell proliferation and differentiation. B Human fetal osteoblastic cell line (hFob) viability was quantified from live/dead cell ratio cultured on various collagen scaffolds and tissue culture plate (TCP) (Mean ± SD, n = 3). C Calcium deposition on various collagen mats by ARS (Alizarin Red S) staining with scale bar = 50 μm. (a) TCP, (b) Pristine collagen mats (ES-Coll), (c) As-spun collagen mats with DA and 20 mM Ca2+ (Coll-DA-Ca), (d) As-spun collagen mats with NE and 20 mM Ca2+ (Coll-NE-Ca), (e) Collagen mats after (NH4)2CO3 exposure (Coll-pDA-Ca), and (f) Coll-pNE-Ca [102]

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