Table 1 Primer sequences

GAPDH

Fw: 5′- GTC AGT GGT GGA CCT GAC CT − 3′

Rv: 5′- TGC TGT AGC CAA ATT CGT TG − 3′

OCT-4

Fw: 5′- GCA GCG ACT ATG CAC AAC GA − 3′

Rv: 5′- CCA GAG TGG TGA CGG AGA CA − 3′

NANOG

Fw: 5′- CTA AGA GGT GGC AGA AAA ACA − 3′

Rv: 5′- CTG GTG GTA GGA AGA GTA AAG G − 3′

SOX-2

Fw: 5′- AGT TGG ACA GGG AGA TGG C − 3′

Rv: 5′- AAC CTT CCT TGC TTC CAC G − 3′

BCL

Fw: 5′- TTT GCT TCA GGG TTT CAT CC − 3′

Rv: 5′- CAG TTG AAG TTG CCG TCA GA − 3′

BAX

Fw: 5′- GGA TTG TGG CCT TCT TTG AG − 3′

Rv: 5′- TAA AGC CAG CCT CCG TTA TC − 3’

RUNX-2

Fw: 5’- GCA GTT CCC AAG CAT TTC AT − 3′

Rv: 5′- CAC TCT GGC TTT GGG AAG AG − 3’

OSX

Fw: 5’- TAA TGG GCT CCT TTC ACC TG − 3′

Rv: 5′- CAC TGG GCA GAC AGT CAG AA − 3’

OCN

Fw: 5’- GTG CAG AGT CCA GCA AAG GT − 3′

Rv: 5′- TCA GCC AAC TCG TCA CAG TC − 3’

OPN

Fw: 5’- TGA AAC GAG TCA GCT GGA TG − 3′

Rv: 5′- TGA AAT TCA TGG CTG TGG AA − 3’