Fig. 7

Osteogenic differentiation of the FS groups. a Alizarin red S staining (scale bar: 100 μm) and (b) a calcium assay (“*”, “#”, and “§” indicate significant differences between groups M, FS-4 and FS-2, respectively) were performed to investigate the amounts of mineralized calcium. c PCR analysis of osteogenic differentiation markers (c) RUNX2, OSX, (d) OCN, and OPN on day 7 (“*”, “#”, and “§” indicate significant differences between groups M, FS-2, and FS-4, respectively)