Fig. 5

The stemness of FS-1, FS-2, and FS-4 groups. PCR analysis of the expression of (a) Nanog, (b) OCT4, and (c) SOX2 as compared to two dimensionally seeded cells (M) on day 7. d Immunostaining of all stemness markers from cross-sectioned FS-2 and group M spheroids (scale bar: 100 μm)