Fig. 1

Preparation of NSC-derived ECM and characterization. Human nasal septal chondrocyte derived extracellular matrix (N-CHDM) is obtained from decellularization of in vitro cultured nasal septal chondrocytes on TCP. Cell morphology (a) before and (b) after decellularization. The inset images are DAPI staining that demonstrates the clearance of cell nucleic components after decellularization. The identity of ECM macromolecules, c type 2 collagen (COL II) and (d) fibronectin (FN), as assessed via immunofluorescence staining. The scale bar is 200 μm. Observation of the surface morphology of (e) N-CHDM and (f) S-CHDM via SEM (The scale bar is 100 μm)