Organ Test | MS Diameter [μm] | No. of Tests | Lungs [%Total] | Liver [%Total] | Body [%Total] |
|---|
Normal Liver | 30 | 3 | 0.07 ± 0.03 | 98.9 ± 0.3 | 0.97 ± 0.23 |
8 | 2 | 0.57 ± 0.35 | 95.2 ± 2.4 | 4.1 ± 2.0 |
Soluble | 2 | 4.7 ± 0 | 15.4 ± 3.0 | 78.1 ± 3.2 |
VX2 Liver | 30 | 2 | 0.02 ± 0.03 | 99.2 ± 0.2 | 0.78 ± 0.18 |
8 | 4 | 0.62 ± 0.07 | 95.7 ± 0.5 | 3.5 ± 0.5 |
Soluble | 2 | 1.83 ± 0.13 | 4.5 ± 1.0 | 93.1 ± 0.95 |
- Polystyrene sulfonate MS (15 mg, 30 μm and 8 μm median diameter) were radiolabelled with 67Ga (avg. 110 MBq) using TA immobilisation. Suspensions of the MS in 5% dextrose (5 mL) were instilled into the hepatic artery of anaesthetised rabbits. Instillation of soluble 67Ga (avg. 110 MBq) was also performed to provide a comparison of the biodistribution. The rabbits were euthanized after 1 h for dissection and gamma camera imaging of lungs, liver, and body. The activities of all images were corrected for background and the activity of each organ expressed as a percentage of the total activity. Note pronounced liver retention of radiolabel using both 67GaTA-MS30 and 67GaTA-MS8 compared to liver retention of soluble 67Ga. Retention of microsphere radiolabel in livers with VX2 tumour implants was indistinguishable from retention by normal livers
