Fig. 9
From: In vivo bone regeneration assessment of offset and gradient melt electrowritten (MEW) PCL scaffolds
IHC analysis of Bmp-2 in calvarial defects from rats 4 and 8 weeks post-implantation of MEW PCL scaffolds. a) Blank (defect control without implanted scaffold), 250 μm, 500 μm. b) Offset.50.50, Grad.750top, Grad.250top. Bmp-2: bone morphogenic protein-2 staining, Iso: Isotype control. The white bar represents 200 μm. c) Quantitative analysis at weeks 4 and 8. The values expressed are the mean ± SD (n = 3). Statistically significant differences between Bmp-2 intensity scores for the treatment groups when compared to each other were determined by ANOVA (Significant: * p < 0.0001, ^ p = 0.009, ■ p = 0.005, ♦ p = 0.002, ▼p = 0.005, ● p < 0.0001)