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Fig. 9 | Biomaterials Research

Fig. 9

From: Role of offset and gradient architectures of 3-D melt electrowritten scaffold on differentiation and mineralization of osteoblasts

Fig. 9

Immunocytochemistry analysis of osteogenitor markers (collagen I, osteopontin and osteocalcin) for human osteoblast cells cultured in: (a, b, c, d) Osteogenic medium (d, e, f) Basal medium after 14 and 30 days; a: 250 μm; b: 500 μm; c: 750 μm; d: Offset.30.70. Merge: Hoechst staining of the nucleus (blue), staining of the antibodies (green fluorescence); (Scale bar = 100 μm)

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