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Fig. 3 | Biomaterials Research

Fig. 3

From: Increased pore size of scaffolds improves coating efficiency with sulfated hyaluronan and mineralization capacity of osteoblasts

Fig. 3

Characterization of aECM modification on scaffolds. The amount of collagen (coll) and high-sulfated hyaluronan (sHA3) immobilized on low (LoPo) and high porosity (HiPo) scaffolds was detected after one hour at 37 °C and after 7 days at 4 °C. a Collagen content was analyzed by o-phthaldialdehyde (OPA) assay and b amount of bound sHA3 by Toluidine blue assay. c Qualitative analysis of collagen and sHA3 was performed using Sirius red and Toluidine blue, respectively. Data represent the mean ± SD. Statistical analysis was performed by two-way ANOVA for the effect of scaffold and time and the interaction (scaffold*time). Scale bar: 5 mm

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