Fig. 1

Summary of key process parameters before and after process optimization to enhance scaffold pore sizes. Cross-sections of the (a) initial LoPo (low porosity) and ( b) optimized HiPo (high porosity) formulation are shown. Lipid content given as mass relative to combined mass of macromer and PEG-MA. (c-f) Lateral view of representative scaffold produced beyond the optimized values. (c) 2x Lipid (75:25 – high:low melting range), shows effect of solely increased lipid amount; 4 g/mL, 50 °C; (d) 2x Lipid (60:40), 2 g/mL, 50 °C, shows effect of the improved lipid/macromer amounts with lower melting range particles; (e) 2x Lipid (50:50), 2 g/mL, 55 °C, shows effect of improved lipid/macromer amounts with increased temperature; (f) 1.75x Lipid (50:50), 3 g/mL, 58 °C, shows effect of optimized formulation with too high temperature. Further details regarding the tuning were compiled in the supporting information. Scale bar = 1.000 μm