Fig. 5

Vascularization after 8 weeks implantation. a Blood vessel density; one-way ANOVA and Tukey’s post-hoc test (n = 18; p < 0.05). Different letters indicate statistically significant differences. b-f Representative H&E stained cryosections showing blood vessels (black arrows). (G-K) Representative immunofluorescent stained sections showing CD31 (red) with DAPI counterstain (blue). White arrows denote blood vessels. (L) CD31 (red) and DAPI (blue) immunofluorescent staining overlaid on a matched bright field image showing the presence of erythrocytes within the vessel lumen