Fig. 4
From: Hypoxic condition enhances chondrogenesis in synovium-derived mesenchymal stem cells
Hypoxic culture enhances chondrogenesis in SDSCs. Histological analysis of chondrogenic cultures of SDSCs. SDSCs (aliquots of 2.0 × 105) were pelleted and induced in chondrogenic differentiation medium under normoxic (21% O2) and hypoxic (5% O2) conditions for 21 days. (a) H&E staining, Safranin-O staining for proteoglycan, and immunohistochemical staining for collagen type II after chondrogenic differentiation for 21 days under normoxic and hypoxic culture conditions. Right panel is high magnification images (b) Western blot of collagen II and X (c) Quantification of glycosaminoglycan (GAG) contents