Fig. 5
From: Antibody-mediated oral delivery of therapeutic DNA for type 2 diabetes mellitus
Cellular transport of hIgG1-Fc-9Arg/pDNA complexes across the Caco-2 cell monolayers. a Cell permeability assay in the Caco-2 monolayer to identify permeability of the complexes through the Caco-2 monolayer. Quantification of fluorescence intensity emitted from the bobo-3 pDNA in the complex indicated cellular transport of the complex from the apical to the basolateral site. b Cell images from confocal analysis of Caco-2 monolayers obtained after treatment of the hIgG1-Fc-9Arg/pDNA complexes. Cells were fixed with 4% PFA. Scale bar is 50 μm