Fig. 4

Relative cellular toxicity of hIgG1-Fc-9Arg/pDNA complexes. Caco-2 cells were incubated with hIgG1-Fc-9Arg/pDNA for 24 h. a MTT assay to estimate the cellular toxicity of hIgG1-Fc-9Arg protein to Caco-2 cells as a delivery vehicle. Mean ± SD (n = 5), 2-way ANOVA with Bonferroni’s multiple comparisons test. # was P < 0.05. b The estimation of the relative cellular toxicity of hIgG1-Fc-9Arg/pDNA complex evaluated by MTT assay. Relative cell viability indicated cellular toxicity of the complex. Relative cell viability obtained from the treatment with hIgG1-Fc-9Arg/pDNA complex was compared with that of cationic polymers (bPEI and lipofectamine). Mean ± SD (n = 5), 2-way ANOVA with Tukey’s multiple comparisons test. # was P < 0.05