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Fig. 3 | Biomaterials Research

Fig. 3

From: Antibody-mediated oral delivery of therapeutic DNA for type 2 diabetes mellitus

Fig. 3

FcRn-mediated cellular uptake of hIgG1-Fc-9Arg/pDNA complex. a FcRn expression in human epithelial colorectal adenocarcinoma cell lines (Caco-2, HT-29), human embryonic kidney cell line (HEK 293), human cervical carcinoma cell line (HeLa), and HEK293-FcRn as a positive FcRn expression control. Representative image from immune- blots of total cellular protein. b Confocal microscopic images of HeLa, HEK293 and Caco-2 cells incubated with of 10 μg of FITC-hIgG1-Fc-9Arg for 1 h. c GFP expression in Caco-2 cells. Different ratios of hIgG1-Fc-9Arg/pAcGFP-N1 complexes were treated for 48 h. All cells were fixed after incubation with 4% PFA. Scale bar is 50 μm

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