Fig. 1
From: Antibody-mediated oral delivery of therapeutic DNA for type 2 diabetes mellitus
Characterization of hIgG1-Fc-9Arg/pDNA complex. a DNA retardation assay to confirm the complexation of hIgG1-Fc-9Arg with pAcGFP-N1. b AFM analysis to observe the surface structure of hIgG1-Fc-9Arg/pAcGFP-N1 complex in double distilled H2O (ddH2O) of pH 7.0. (1) hIgG1-Fc-9Arg only, (2) 20/1 weight ratio of hIgG1-Fc-9Arg/ pAcGFP-N1 complex, (3–4) 50/1 weight ratio of hIgG1-Fc-9Arg/pAcGFP-N1 complex, and (5) 100/1 weight ratio of hIgG1-Fc-9Arg/pAcGFP-N1 complex. Scale bar represents 200 nm. c FE-TEM analysis to observe the structure of hIgG1-Fc-9Arg/pAcGFP-N1 complex. The complex ratios are (a) (20/1, w/w), (b) (50/1, w/w), (c) (100/1 w/w). Scale bar indicates 100 nm. d The mean size of hIgG1-Fc-9Arg/ pAcGFP-N1 complex measured by FE-TEM. Mean ± S.D. from at least ten separate images of the complex