Fig. 2
From: Schwann cell durotaxis can be guided by physiologically relevant stiffness gradients
a, b Relative to Schwann cells cultured on a uniform substrates (4325 Pa), Schwann cells cultured on b steep gradient (243-4325 Pa) substrates had increased expression of paxillin (red), which co-localized to actin staining (green), indicating increased focal adhesion formation. Scale bar represents 100 μm. c, d Scanning electron micrographs of Schwann cells adherent to substrates are shown at 450x magnification (scale bar is 50 μm) and 20 degree tilt; inset 1500x magnification (scale bar is 10 μm). Schwann cell on c uniform substrate exhibits intact plasma membrane surface. Schwann cell on d steep gradient exhibits plasma membrane morphology characterized by vesicles (arrows) fusing with the plasma membrane