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Table 2 Primer sequence for RT-PCR

From: The effect of serum types on Chondrogenic differentiation of adipose-derived stem cells

Gene

Forward(5′- > 3′)

Reverse(5′- > 3′)

Col I

CTC CGG CTC CTG CTC CTC TTA

GCA CAG CAC TCG CCC TCC C

Col II

ATA AGG ATG TGT GGA AGC CG

TTT CTG TCC CTT TGG TCC TG

Aggrecan

TTG AGC AGT TCA CCT TC

CTC TTC TAC GGG GAC AGC AG

Sox-9

CCC AAC GCC ATC TTC AAG G

CTG CTC AGC TCG CCG ATG T

GAPDH

GGG AGC CAA AAG GGT CAT CAT CTC

GAG GGG CCA TCC ACA GTC TTC

  1. Primer sequences for RT-PCR using chondrogenic gene markers. Collagen type II, Aggrecan and Sox-9 were used for chondrogenic markers with using collagen type I as a negative gene marker. GAPDH was used as a housekeeping gene
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Biomaterials Research

ISSN: 2055-7124

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