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Table 2 Primer sequence for RT-PCR

From: The effect of serum types on Chondrogenic differentiation of adipose-derived stem cells

Gene Forward(5′- > 3′) Reverse(5′- > 3′)
Col I CTC CGG CTC CTG CTC CTC TTA GCA CAG CAC TCG CCC TCC C
Col II ATA AGG ATG TGT GGA AGC CG TTT CTG TCC CTT TGG TCC TG
Aggrecan TTG AGC AGT TCA CCT TC CTC TTC TAC GGG GAC AGC AG
Sox-9 CCC AAC GCC ATC TTC AAG G CTG CTC AGC TCG CCG ATG T
GAPDH GGG AGC CAA AAG GGT CAT CAT CTC GAG GGG CCA TCC ACA GTC TTC
  1. Primer sequences for RT-PCR using chondrogenic gene markers. Collagen type II, Aggrecan and Sox-9 were used for chondrogenic markers with using collagen type I as a negative gene marker. GAPDH was used as a housekeeping gene