Fig. 4

Alizarin red S staining and quantification on GM-ECM, OD-ECM-2 W, and OD-ECM-4 W for 2 and 4 weeks with growth media or osteogenic differentiation media. a Representative images of Alizarin red S staining with each decellularized ECMs and re-seeded osteoblast. The scale bar represents 400 μm. b Quantification of Alizarin red S with each group. ^*p < 0.05 compared to GM-ECM, ^#p < 0.05 compared to OD-ECM-2 W. CTL, control; GM, growth medium; ODM, osteogenic differentiation medium