Fig. 2

Representative optical microscope images of cultured osteoblast and re-seeded osteoblast on decellularized ECM with various conditions. a Osteoblasts were cultured with growth medium for 3 days (GM-Osteo) before decellularization. After decellularized ECM (GM-ECM). Osteoblasts were re-seeded on GM-ECM. Osteoblasts were cultured with osteogenic differentiation medium for (b) 2 weeks (OD-Osteo (2 Wks)) and (c) 4 weeks (OD-Osteo (4 Wks)). After decellularized ECM (OD-ECM-2 W and OD-ECM-4 W). Osteoblasts were re-seeded on decellularized OD-ECM. The scale bar represents 400 μm