Table 1 List of primer sequences for RT-PCR analysis

GADPH

F: 5′-tcc ctg agc tga acg gga ag-3′

R: 5′-gga gga gtg ggt gtc gtc gct gt-3′

NM_002046

Human Collagen Type I, COL I

F: 5′-agg gct cca acg aga tcg aga-3′

R: 5′-tac agg aag cag aca ggg cca-3′

NM_000088

Human Collagen Type II, COL II

F: 5′-cta tct gga cga agc agc tgg ca-3′

R: 5′-atg ggt gca atg tca atg atg g-3′

NM_001844

Human Aggrecan Core Protein, ACP

F: 5′-cac tgt tac cgc cac ttc cc-3′

R: 5′-acc agc gga agt ccc ctt cg-3′

NM_001135

Human Transcription Factor SOX-9, SOX-9

F: 5′-gcg gag gaa gtc ggt gaa ga-3′

R: 5′-ccc tct cgc ttc agg tca gc-3′

NM_000346

Homeobox Transcription Factor, NANOG

F: 5′- ctg tga ttt gtg ggc ctg aa −3′

R: 5′- tgt ttg cct ttg gga ctg gt-3′

NM_024865

SRY-related HMG-Box-2, SOX-2

F: 5′- tta cct ctt cct ccc act cca −3′

R: 5′- ggt agt gct ggg aca tgt gaa-3’

NM_003106

Reduced Expression-1, REX-1

F: 5′-aaa ggt ttt cga agc aag ctc-3′

R: 5′-ctg cga gct gtt tag gat ctg-3′

NM_174900

Octamer-Binding Protein 3/4, OCT-4

F: 5′- aag gat gtg gtc cga gtg tg-3′

R: 5′- gaa gtg agg gct ccc ata gc-3′

NM_002701

Osteocalcin, OSC

F: 5′-gtg cag agt cca gca aag gt −3′

R: 5′-tca gcc aac tcg tca cag tc −3′

NM_199173

Osteopontin, OSP

F: 5′-tga aac gag tca gct gga tg-3′

R: 5′-tga aat tca tgg ctg tgg aa-3′

NM_001040060

Human Collagen Type X, COL X

F: 5′-gct aag ggt gaa agg ggt tc-3′

R: 5′-ctc cag gat cac ctt ttg ga-3′

NM_000493