Fig. 5

ALP activity of hMSC cultured on CaP-A, CaP-I with 0, 1, 5 and 10 mM of Si and CaP-M with 0, and 1 mM of Si after 3, 7 and 14 days. OM represent the condition in which the cells were cultured on CaP coatings in osteogenic medium. Significant differences among different conditions analyzed at the same time point are indicated by asterisks + patterned lines. Connector lines + asterisk indicate the significant differences in the same condition between different time points. (p < 0.05; n = 6). Values were normalized to μg of DNA. ALP expression was remarkably higher in CaP-I coatings. Cells cultured on CaP-I 5 mM and 10 mM showed a higher ALP activity than the other conditions. CaP-A 10 mM showed a higher ALP expression than CaP-A 1 mM and 5 mM Si after 14 days of culture