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Table 1 Physical properties of PVA-Tyr hudrogels crosslinked via varying enzyme types and concentrations

From: A comparative study of enzyme initiators for crosslinking phenol-functionalized hydrogels for cell encapsulation

Enzyme Oxidant Time to gel (min)a Final absorbance at gelation (325 nm)b Sol fraction (% mass loss at 48 h) Swelling (q, at 48 h) Crosslinking density (ρx, mmol/L)c Dynamic shear modulus (G*, Pa)
Name Concentration Unit Name Concentration Unit
HRP 0.1 U/mL H2O2 6 mM 20 1.4 ± 0.2 32 ± 7 % 29 ± 3 2.2 138 ± 43
0.2 6 15 2.0 ± 0.2 31 ± 5 % 23 ± 1 3.8 219 ± 30
0.2 12 20 1.2 ± 0.1 36 ± 7 % 33 ± 5 1.6 89 ± 24
Hematin 0.01 % (w/w) H2O2 6 mM 60 0.3 ± 0.0 76 ± 8 % 168 ± 36 0.1 5 ± 1
0.06 6 15 0.7 ± 0.2 63 ± 10 % 121 ± 25 0.1 11 ± 3
0.06 12 30 1.1 ± 0.1 49 ± 11 % 84 ± 16 0.2 38 ± 5
Laccase 5 U/mL O2    360+ 2.4 ± 0.2 33 ± 6 % 26 ± 4 2.8 97 ± 34
15    240 2.9 ± 0.1 24 ± 10 % 21 ± 3 4.6 145 ± 22
Tyrosinase 500 U/mL O2    150 1.7 ± 0.1 26 ± 5 % 44 ± 7 0.9 145 ± 49
750    150 2.0 ± 0.2 20 ± 8 % 35 ± 10 1.4 169 ± 56
  1. aGelation time was determined as the time when there was no further significant increase in absorbance
  2. bThe absorbance after the hydrogel had completed gelation as determined by the time to gel
  3. cCrosslinking density was determined from swelling (q) at 48 h using equations derived from Flory and Rehner [30]