Fig. 6

Representative confocal microscopy images of fibroblast viability within in PVA–Tyr hydrogels immediately after 4 h of gelation (a, b, c) or after 7 days of culture (d, e, f). PVA–Tyr hydrogels were polymerized with different enzyme initiators; A,D) HRP (0.2 U/mL) with H₂O₂ (6 mM), b, e) laccase (15 U/mL), and c, f) tyrosinase (750 U/mL). Live cells fluoresce green and dead cells fluoresce red. Scale bars represent 200 μm. g Fibroblast cell number was determined quantitatively over 7 days within the PVA-Tyr hydrogels by the MTS assay (absorbance at 490 nm). The lines between data points are meant to guide the eye in observing the trends of the data