Fig. 4
From: Multiple genetically engineered humanized microenvironments in a single mouse
Analysis of growth-arrested engineered stromal cell type dependent tissue microenvironments. a Schematic of experiment. Growth-arrested genetically engineered stromal cells were co-seeded with hBMSCs into hydrogel scaffolds and subdermally implanted for 6 weeks. Explanted scaffolds were characterized for collagen deposition and vasculature development. b Masson’s Trichrome staining visualizes collagen fibers (Blue). c Semi-quantitative analysis of collagen deposited area (N = 6). d Immunohistostaining of mouse CD31 (green) and nucleus (DAPI) identifies inter-scaffold vasculatures. e Semi-quantitative analysis of vasculature area (N = 6). (Scale bar, 200 μm) (*P < 0.01, **P < 0.05)