Fig. 1
From: Multiple genetically engineered humanized microenvironments in a single mouse
Creating genetically engineered stromal cell-coated implantable microenvironments. a Design of lentiviral vectors encoding hVEGFa, hSDF1a, and hTNFa genes for genetically engineered mBMSC-line generation. b Microfabricated hydrogel scaffold that represents a standardized and fully interconnected porous microstructure (top) and a fluorescent image of genetically engineered mBMSC residing in a 3D scaffold (bottom). c-d Normalized secretion of (c) hVEGFa, (d) hSDF1a, and (e) hTNFa from genetically engineered stromal cells for 3Â days. The secretion rates were compared with hBMSC growing in the same hydrogel scaffolds