Fig. 2

Cyclic tensile stretching parallel to the micro-patterned cell lines (0° stretch) results in irregular myoblast orientation. (Left) The cell stretching device in which cell adhesive lines were oriented at 0°, 45°, and 90° to the stretch direction. (Right) a-f Actin stress fiber orientation in C2C12 myoblasts. Unstretched controls on HS (homogenous surface) and line patterns are shown. Cells exposed to cyclic tensile stretching (CTS) on HS are shown (stretch direction is horizontal). CTS applied for cell line patterns at varying stretch angles are also shown (reprinted from Ahmed et al. [31] with permission from Elsevier)