Table 2 Real time-qPCR primer and product size
Gene | Sequence (5′ → 3′) | Size (bp) | Cycle | Annealing temp. | Ref. | |
|---|---|---|---|---|---|---|
β-Actin | (S) | ACAGAGCCGCCTCTGCC | 124 | 45 | 58 °C | Genebank AB009345 |
(AS) | ACAAGCCTGAGCCGTTGTC | |||||
BSP | (S) | ATACCATCTCACACTAGTTATAATG | 116 | 45 | 58 °C | Genebank NM000493 |
(AS) | AACAGCATAAAAGTGTTCCTATATC | |||||
COLI | (S) | GCAAGAGAGAAAAGAGTGAACC | 103 | 45 | 58 °C | Genebank AY633663 |
(AS) | GTGGCTCAAGCAGAACCAG | |||||
Cbfa-1 | (S) | CAGTCACATCAGGATATCC | 117 | 45 | 58 °C | Genebank L38480 |
(AS) | ATGCTGCTGATCTGGAAGA | |||||
OCN | (S) | CTCCAGGCACCCATCTTTAC | 121 | 45 | 58 °C | Genebank NM00095 |