Table 1 PCR primer and product size
Gene | Sequence (5′ → 3′) | Size (bp) | Cycle | Annealing temp. | Ref. | |
|---|---|---|---|---|---|---|
COLI | (S) | AGAACATCACCTACCACTGC | 250 | 35 | 58 °C | Genebank AY633663 |
(AS) | ATGTCCAAAGGTGCAATATC | |||||
Cbfa-1 | (S) | AGAGGTACCAGATGGGACTGTGGTT | 316 | 35 | 61 °C | Genebank S83370 |
(AS) | GGTAGCTACTTGGGGAGGATTTGTG | |||||
BSP | (S) | CAATAGTGACTCATCCGAAG | 280 | 35 | 55 °C | Genebank Z46629 |
(AS) | CTCCTCATCTTATTCATCAC | |||||
GAPDH | (S) | TCACAATCTTCCAGGAGCGA | 293 | 35 | 58 °C | Genebank L23961 |
(AS) | CACAATGCCGAAGTGGTCGT | |||||