Fig. 5

Immunofluorescence images of C2C12 cells in GHPA hydrogels. 2D fluorescence images of C2C12 myoblasts in GM (a-c) and DM (h-j) and MPM images of C2C12 myoblasts in GM (d-g) and DM (k–n). The F-actins were stained with TRITC-labeled phalloidin (red) and the MHCs were stained with Alexa Fluor 488-conjugated anti-MHC monoclonal antibody (green). All images shown in this figure are representative of six independent experiments with similar results