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Fig. 2 | Biomaterials Research

Fig. 2

From: Investigation of the changes of biophysical/mechanical characteristics of differentiating preosteoblasts in vitro

Fig. 2

Osteogenic induction of preosteoblasts on gelatin matrices. Immunofluorescence images of a, e type I collagen and b, f osteocalcin at day 7 and 14, respectively. c, g) Alizarin red and d, h von Kossa staining of preosteoblasts at day 7 and 14, respectively. Quantification of i alizarin red, j von kossa positively-stained area, k normalized ALP activity, and l calcium content, respectively. Results are mean ± SD of one triplicate approach that is representative of three independent experiments. *** p < 0.001 indicates a statistically significant difference

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Biomaterials Research

ISSN: 2055-7124

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