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Fig. 4 | Biomaterials Research

Fig. 4

From: Biocompatibility of a polymer based on Off-Stoichiometry Thiol-Enes + Epoxy (OSTE+) for neural implants

Fig. 4

a Results from MTT assay tests using the L929 cell line. The L929 cells were seeded and allowed to attach in the wells of 96-well plates for 24 h. The cells were then subjected to extraction solutions of OSTE+, OSTE+H2O, polyimide and HDPE for 72 h at different concentrations (6 %, 12.5 %, 25 % and 50 %). DMSO at 2.5 % was used as a positive control. The results are presented as mean values (n = 18–36) and the error bars represent ± SEM. The data was analyzed using a 2-way ANOVA, comparing the data for each set vs. control where ** represents a P-value ≤ 0.01 and **** represents a P-value ≤ 0.0001. b The L929 cells were seeded and after 24 h treated for 72 h with the chemicals that were used in the production of OSTE+. Cell viability was assessed using the MTT assay. The results are presented as mean values (n = 6–18) and the bars represent ± SEM. All chemicals, used as single agents, are non-toxic at concentrations below 1 μg/ml. c Phase contrast (top row) and confocal microscopy (bottom row) images of mouse L929 cells exposed to extracts of OSTE+, OSTE+H2O, polyimide and HDPE at a 50 % concentration for 72 h. The phase contrast images show viable and non-stained cells whereas the cells visualized with confocal microscopy were fixed and stained to show the actin cytoskeleton (red) and the nuclei (blue). The scale bars are equal to 50 μm. Images shown were taken in areas with similar cell densities

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