Fig. 4

In vitro cellular biocompatibility of the scaffold. The biocompartibility was evaluated using an immortalized human umbilical vein cell line (EA.Hy926). Cells were cultured on each scaffold and proliferated gradually over time on all scaffolds. However, the proliferation was the highest on the FB scaffold loaded with 2 μg of ANG (FB/ANG 2.0) (a). After 3 days of cultivation, live/dead staining showed that most of the cells were viable (stained green by Calcein AM), whereas dead cells (stained red by EthD-1) were not observed. Scale bar = 500 μm (b). To evaluate cell adhesion, scanning electron microscopy (SEM) (C) was performed after 5 days of cultivation. SEM images showed that cell adhesion increased as ANG content increased (c). The data shown are the mean ± SD of three independent experiments. * and # indicate significantly different when compared with the FB scaffold and FB/ANG 0.5 scaffold, respectively