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Table 1 Common bacterial species found in endodontic root canals and culture condition

From: Evaluation of ciprofloxacin and metronidazole encapsulated biomimetic nanomatrix gel on Enterococcus faecalis and Treponema denticola

Bacterial species Source Media Condition
Liquid Solid
A. actinomycetemcomitans Y4 BHI broth BHI + agar 37°C, 5% CO2
F. nucleatum ATCC 23726 BHI broth BHI + agar 37°C, Anaerobic
P. endodontalis ATCC 35496 Pg broth Blood agar 37°C, Anaerobic
P. gingivalis 2561 Pg broth Blood agar 37°C, Anaerobic
P. nigrescens ATCC 33563 Pg broth Blood agar 37°C, Anaerobic
P. intermedia ATCC 25611 Pg broth Blood agar 37°C, Anaerobic
T. forsythia ATCC 43037 PY borth NAM medium 37°C, Anaerobic
T. denticola ATCC 3521 TYGVS TYGVS + agar 37°C, Anaerobic
E. faecalis ATCC 4083 BHI broth BHI + agar 37°C, Facultative
L. casei HY 2782 MRS MRS + agar 37°C, Facultative
S. gordonii G9B BHI broth BHI + agar 37°C, Facultative
S. mutans GS5 BHI broth BHI + agar 37°C, Facultative
S. sobrinus 6715 BHI broth BHI + agar 37°C, Facultative
S. sanguinis clinical isolates BHI broth BHI + agar 37°C, Facultative
  1. BHI: Brain-heart infusion, Pg: Porphyromonas gingivalis , TYGVS: tryptone-yeast extract-gelatin-volatile fatty acids-serum, MRS: deMan, Rogosa and Sharpe, NAM: N-acetylmuramic acid.