Immunostaining of actin cytoskeleton. Actin cytoskeleton was stained with Alexa (488)-conjugated phalloidin (green) under static and flow condition. (A) Under static condition (B) Shear stress of 8 dyne/cm2 was applied for 30 minutes (C) Shear stress of 8 dyne/cm2 was applied for 5 hours. Flow direction is marked by arrows. Scale bar = 50 μm.