Figure 5
From: Immobilizing hydroxycholesterol with apatite on titanium surfaces to induce ossification
Effects of adsorbed apatite/20α-HC coating on mineral deposition in rat model. Experimental groups were set up: (A) CaP-Ti discs were immersed in DPBS solution for 24 h to prepare apatite coating. Apatite coated CaP-Ti discs were immersed in 100% ethanol containing (B) 1 mg/mL, (C) 2 mg/mL and (D) 4 mg/mL 20α-HC for 10 s and then dried in air to prepare adsorbed apatite/20α-HC coating. All the experiment groups were subcutaneously implanted into rat muscle bed for 5 weeks, and then the deposited mineral on surfaces of samples were stained with Alizarin Red S.