Fig. 9

Bone regeneration by transplantation of TMP cryogels in vivo. (a) Schematic illustration of TMP/Gel implantation into mouse calvarial defects and micro-CT results (scale bar = 1 mm). (b) The regenerative bone volume (BV/TV), bone area (right), and other values associated with newly formed bone tissue, Tb.Th., Tb.Sp., and Tb.N., as determined by analyzing the micro-CT images (*, significant difference, P < 0.05) (n = 5). (c) H&E staining and Goldner’s trichrome images of mouse calvarial defects (scale bar = 500 μm) and enlarged images of histological sections of the TMP/Gel (scale bar = 100 μm). (d) Immunohistochemistry images of tissue specimens implanted with MP/Gel and TMP/Gel stained for TNF-α (green) (scale bar = 250 μm). (e) Immunohistochemistry images of tissue specimens implanted with TMP/Gel and stained for α-SMA (green) (scale bar = 250 μm), with an enlarged image (scale bar = 100 μm)