Table 1 The gene primer sequences for qPCR
|  | Primer | Sequences (5’-3’) |
|---|---|---|
Mouse Zip1 | Zip1-F | CTGGACCTGCTGCCTGACTAC |
Zip1-R | TGCCCAGTAGAGCCCTTGTC | |
Mouse Zip2 | Zip2-F | ACTGGGCTGTGGCCTTACTC |
Zip2-R | CTTCCTGTACTATTCTGTTCCACG | |
Mouse Zip3 | Zip3-F | TGCCTGCAGTGAGGGACAAG |
Zip3-R | ATCTGAGCCGGCGTTGAAGG | |
Mouse Zip4 | Zip4-F | GGGCCGTGTGAAAAGTGTC |
Zip4-R | TTAAGGTAGAGGGCAGCAGC | |
Mouse Zip5 | Zip5-F | CCTAGCAGTCTTCTGCCACGAG |
Zip5-R | GAAGCAGGGTTGGCAGCATATC | |
Mouse Zip6 | Zip6-F | GAGAGCAAGAAGCAGCTGTCC |
Zip6-R | ATCGTCGGCTGCTGGGAATC | |
Mouse Zip7 | Zip7-F | GGGCCACAGTGCTGATCTCC |
Zip7-R | GGTGAGAATGAGGTTCCAAGGC | |
Mouse Zip8 | Zip8-F | TCGCCTTCAGTGAGGATGTGC |
Zip8-R | TGTAAGATCGCGGGGCAGATG | |
Mouse Zip9 | Zip9-F | TGCTGGTCTGGAGCGGAATC |
Zip9-R | GTGGCTGTGCCCCATTCCA | |
Mouse Zip10 | Zip10-F | TGGTGATCATGGGTGACGGC |
Zip10-R | TGCTTTACGGTCATGCCTGC | |
Mouse Zip11 | Zip11-F | CCGGATAGGTAGCACTGGGC |
Zip11-R | CGCCAACAGCAAGACCCTCT | |
Mouse Zip12 | Zip12-F | CCTGGTTGGAGATAGCCTGCAC |
Zip12-R | AAGGCCGTCAGGGCACTTAG | |
Mouse Zip13 | Zip13-F | GGGCTCTCTCATGGTTGGGC |
Zip13-R | GGCGGGGGTGATGTTACAGG | |
Mouse Zip14 | Zip14-F | GCGTTTTTCAGCCGTGTGCT |
Zip14-R | TATGCCCGTGATGGTGCTCG | |
Mouse Runx2 | Runx2-F | CGAACAGAGCAACATCTCC |
Runx2-R | GTCAGTGCCTTTCCTTGG | |
Mouse Alpl | Alpl-F | TGCTACCTGCCTCACTTCC |
Alpl-R | GAGAGAAACCCACCCTGCT | |
Mouse Osterix | Osterix-F | GGCCTTTCGTCTGCAACT |
Osterix-R | TGCTCAAGTGGTCGCTTC | |
Mouse Gapdh | Gapdh-F | TGTGTCCGTCGTGGATCTGA |
Gapdh-R | TTGCTGTTGAAGTCGCAGGAG | |
Rat ZIP4 | ZIP4-F | ATGAGCTGCCTCACGAACTT |
ZIP4-R | CTGCTAGAGCCACGTAGAGG | |
Rat RUNX2 | RUNX2-F | GACTGTGGTTACCGTCATGGC |
RUNX2-R | ACTTGGTTTTTCATAACAGCGGA | |
Rat ALPL | ALPL-F | CCAACTCTTTTGTGCCAGAGA |
ALPL-R | GGCTACATTGGTGTTGAGCTTTT | |
Rat OSTERIX | OSTERIX-F | GGAAAGGAGGCACAAAGAAGC |
OSTERIX-R | CCCCTTAGGCACTAGGAGC | |
Rat GAPDH | GAPDH-F | CAAGAAGGTGGTGAAGCAG |
GAPDH-R | CAAAGGTGGAAGAATGGG |